U1ii: Describe the role of insulin in fat metabolism
Insulin Summary
- Polypeptide Hormone
- Stored + synthesised by β cells of pancreas
- Ca2+ required for exocytosis & release
- Released when BSL > 6mmol/L
- Binds Insulin Receptor A subunits
- Activates TK
- Autophosphorylation of receptor
- Alters intracellular gene transcription
- Major ANABOLIC HORMONE
Fat Metabolism
Adipose Tissue
- ↑fat synthesis
- ↑glucose uptake via GLUT-4
- Glycolysis: glucose → Acetyl CoA
- One Acetyl CoA has 2-C → add additional Acetyl CoA until you have FA of appropriate length → storage as TAGs
↑ Fat Absorption
- LIPOPROTEIN LIPASE (LPL) is synthesised by skeletal m. + AT
- ↑[Insulin] post meal → ↑AT LPL secretion
- LPL hydrolyses TAGs → release Glycerol + FAs
→ Taken up by tissues for E. oxidation / re-esterification & storage
↓ Fat Breakdown
- Inhibits HORMONE SENSITIVE LIPASE (HSL)
- HSL breaks stored TAGs → glycerol + FFA → circulation
- Inhibits ketogenesis
Liver
↑ Fat Synthesis
- ↑glucose entry via GLUT-4 (but not required v. perm to CHO)
- Glucose converted to glycogen
- When glycogen stores maximal (100g), excess glucose → pyruvate → Acetyl CoA → stored TAGs
↓ Fat Breakdown
- Inhibits gluconeogenesis
- Inhibits ketoneogenesis
Skeletal Muscle
- ↑LIPOPROTEIN LIPASE secretion → breaks down digested fat to release TAGs → Glycerol + FA for storage
- ↓Ketogenesis